Molecular Formula | C14H12N2O4 |
Molar Mass | 272.26 |
Density | 1.374±0.06 g/cm3(Predicted) |
Melting Point | 266-268 °C(Solv: ethyl acetate (141-78-6)) |
Solubility | DMSO: ≥ 29 mg/mL |
pKa | 7.42±0.20(Predicted) |
Storage Condition | -20℃ |
In vitro study | ML346 is an activator of Hsp70, with an EC 50 of 4600 nM in HeLa cells. ML346 (10 μM) restores proteostasis, restores CFTR-mediated iodide conductance, and enhances the correct folding of proteins expressed in two different cellular compartments. ML346 (Compound F1) induces multiple responses and strongly induces Hsp70, the oxidative stress response genes (HO1 and GCLM), and a 2.5-fold upregulation of BiP in WT MEF cells. ML346 (0.5-25 μM) exhibits cytoprotective effects in cells after a 35 min severe heat shock, and also causes a two-fold protection from H 2 O 2 -induced apoptosis. |
In vivo study | ML346 suppress the aggregation of polyQ35 in a C. elegans model, suggesting the probe has efficacy in modifying protein aggregation and associated toxicity. |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 3.673 ml | 18.365 ml | 36.73 ml |
5 mM | 0.735 ml | 3.673 ml | 7.346 ml |
10 mM | 0.367 ml | 1.837 ml | 3.673 ml |
5 mM | 0.073 ml | 0.367 ml | 0.735 ml |
biological activity | ML346 is an activator of Hsp70, a novel regulator of protein steady state. |
Target | Value |